Journal: Molecular cell

Article Title: The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma

doi: 10.1016/j.molcel.2022.03.019

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum , ATCC , Cat# PCS-500-040.

Techniques: Microarray, Recombinant, Sample Purification, Isolation, Derivative Assay, shRNA, Construct, CRISPR, Plasmid Preparation, Software

Journal: Molecular cell

Article Title: The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma

doi: 10.1016/j.molcel.2022.03.019

Figure Lengend Snippet: A. Experimental schematic to profile BAF complex localization and other chromatin changes in hTERT-MSCs upon 24 hours adipogenesis. B. edgeR analysis of differential occupancy of BAF complexes (SMARCA4) on the genome upon 24 hours adipogenesis. C. HOMER motif analysis of sites that gain BAF complex (SMARCA4) occupancy upon 24 hours adipogenesis. D. Heatmap of SMARCA4, CEBPB, H3K27ac, and ATAC-seq peaks over sites that gain BAF complex (SMARCA4) occupancy upon 24 hours adipogenesis (“SMARCA4 targets”). E. Experimental schematic to define the specific role for BAF complexes in adipogenesis using small molecule-mediated inhibition of BAF complex ATPase activity with compound 12 (CMP12). F. Heatmap of SMARCA4, CEBPB, H3K27ac, and ATAC-seq peaks upon DMSO or CMP12 treatment in base or adipogenic media for 24 hours over SMARCA4 gained sites in the DMSO adipogenesis condition. G. Comparison of changes in RNA in the CMP12 and DMSO treatment conditions upon adipogenesis. Dark blue circles are genes that are not as up-regulated or are more down-regulated upon adipogenesis with CMP12 treatment compared to DMSO (CMP12-attenuated gene). Light blue circles are a subset of CMP12-attenuated genes that are also nearest to sites that gain BAF complexes (SMARCA4) upon adipogenesis in the DMSO condition (CMP12-attenuated gene and SMARCA4 target). H. Gene ontology analysis of CMP12-attenuated gene and SMARCA4 targets. I. Heatmap of logFC in expression of genes in DMSO and CMP12 conditions upon adipogenesis. J. Track examples at the CD36, ELOVL6 and WNT5A loci. See also Figure S4.

Article Snippet: Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum , ATCC , Cat# PCS-500-040.

Techniques: Inhibition, Activity Assay, Comparison, Expressing

Journal: Molecular cell

Article Title: The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma

doi: 10.1016/j.molcel.2022.03.019

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low Serum , ATCC , Cat# PCS-500-040.

Techniques: Microarray, Recombinant, Sample Purification, Isolation, Derivative Assay, shRNA, Construct, CRISPR, Plasmid Preparation, Software

Journal: BioMed Research International

Article Title: MicroRNAs as Salivary Markers for Periodontal Diseases: A New Diagnostic Approach?

doi: 10.1155/2016/1027525

Figure Lengend Snippet: Comparison of methods from current investigations regarding miRNAs in periodontal disease.

Article Snippet: Zahran et al. 2015 [ ] , 20 healthy controls, 40 potentially malignant disorders, 20 OSCC, 20 recurrent aphthous stomatitis , Unstimulated saliva supernatant , miRNeasy serum/plasma extraction kit (Qiagen) , — , RT-qPCR SYBR green PCR kit (Qiagen) , SNORD68 , One-way ANOVA, F -test, Dunnett t -test, Scheffe's multiple comparison, two-tailed tests , miR-21, miR-184, miR-145.

Techniques: RNA Extraction, In Vitro, Microarray, Isolation, TaqMan microRNA Assay, SYBR Green Assay, Labeling, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, In Vivo, Expressing, Mann-Whitney U-Test

Journal: BioMed Research International

Article Title: MicroRNAs as Salivary Markers for Periodontal Diseases: A New Diagnostic Approach?

doi: 10.1155/2016/1027525

Figure Lengend Snippet: Comparison of methods for salivary miRNA diagnosis.

Article Snippet: Zahran et al. 2015 [ ] , 20 healthy controls, 40 potentially malignant disorders, 20 OSCC, 20 recurrent aphthous stomatitis , Unstimulated saliva supernatant , miRNeasy serum/plasma extraction kit (Qiagen) , — , RT-qPCR SYBR green PCR kit (Qiagen) , SNORD68 , One-way ANOVA, F -test, Dunnett t -test, Scheffe's multiple comparison, two-tailed tests , miR-21, miR-184, miR-145.

Techniques: Marker, Isolation, Mann-Whitney U-Test, TaqMan microRNA Assay, TLDA Assay, Expressing, Microarray, Lysis, SYBR Green Assay

Journal: Cell stem cell

Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay

doi: 10.1016/j.stem.2017.01.002

Figure Lengend Snippet: (A) HSCs/mL following G-CSF mobilization in wild-type mice treated with saline or Scopolamine hydrobromide (Scop 1mg/kg; n = 5–8). HSCs defined as Lineage− Sca1+ cKit+ Flt3−.

Article Snippet: Microarray analysis RNA for microarray analysis was isolated by sorting HSC (Lineage − Sca1 + cKit + Flt3 − directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions.

Techniques:

Journal: Cell stem cell

Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay

doi: 10.1016/j.stem.2017.01.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Microarray analysis RNA for microarray analysis was isolated by sorting HSC (Lineage − Sca1 + cKit + Flt3 − directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions.

Techniques: Recombinant, Labeling, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Microarray, Sequencing, Software, Imaging